|
KMID : 0364819860240020098
|
|
Korean Journal of Microbiology
1986 Volume.24 No. 2 p.98 ~ p.105
|
|
|
Rhizobium meliloti 102F51 Mutants Defective in Heme Synthesis
|
|
Choi, Y.J/최영주
Chung, W.H/Kim, K.S/Shin, P.G/Cho, M.J/정원화/김경수/신평균/조무제
|
|
|
Abstract
|
|
|
1
Rhizobium meliloti 102F51, the symbiotic partner of alfalfa, was mutagenized with N-methyl-N´-nitro-N-nitrosoguanidine (NTG) and UV-irradiation. Three group of mutants which form white, white-pink and red nodules were selected. The acetylene reduction activity, nodulating activity, amount of heme synthesis during the nodulation, and S-aminolevulinic acid synthetase (ALAS) and S-aminolevulinic acid dehydratase (ALAD) activities in free living rhizobia and bacteroid states of the each group of mutants were compared. The mutants forming white nodules showed lower acetylene reduction activity compared to those of red nodule forming mutants. The two key enzymes for the heme synthetic pathway, ALAS and ALAD activities of the mutants forming red nodules was much higher than those of the mutants forming white nodules in bacteroid state, however no significant difference was observed in free living state. In the nodules the ALAS was detected only in bacteroid fraction, while ALAD was detected both in bacteroid and plant fraction. ALAS was dramatically increased with the heme synthesis during the nodulation, while ALAD was decreased in plant fraction but slight increase was observed in bacteroid fraction.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|